IRIDA provides multiple analysis pipelines for analysing your sequencing data. These pipelines take as input a set of sequencing read files from different samples. The samples must first be added to a cart before a pipeline can be run.
- Adding samples to the cart
- Searching samples in the cart
- Selecting a pipeline
- Modifying pipeline parameters
- Running the pipeline
- Monitoring pipeline progress
- Viewing pipeline results
- Sharing pipeline results
- Saving pipeline results to a sample
- Downloading single sample analysis output files in batch
- Errors when executing pipelines
Adding samples to the cart
The cart is a place where you can temporarily store a selection of samples from across multiple projects for submission to a pipeline for analysis.
You can see the current contents of the cart by clicking on the “Cart” button, on the top, right-hand side of the dashboard:
The cart will appear on the right-hand side of the page:
The cart is initially empty. You can add samples to your cart by navigating to the samples list for a project and selecting the samples that you want to add. Once you’ve selected the samples that you want to add, click on the “Add to Cart” button, just above the samples list:
Once you’ve added some samples to the cart, if you click the “Cart” button on the dashboard, you’ll be able to review all of the samples that you’ve selected, organized by project:
You can remove individual samples or all selected samples from a project by clicking on the icon, or you can empty the cart entirely by clicking on the “Clear” button, in the top, right-hand corner of the cart:
Searching samples in the cart
You can search for individual samples within the cart use the ‘cart search’ field.
Search terms can match partial samples names. Note: projects that do not contain any samples that match the filter are hidden.
Selecting a pipeline
Once you’ve selected the samples that you want to analyse and added them to the cart, you can click on the “Select a Pipeline” button at the bottom of the cart:
The pipelines page will allow you to choose from the pipelines that are currently configured in IRIDA. IRIDA has multiple pipelines:
Click on the “Select” button at the bottom, right-hand side of the pipeline that you’d like to launch.
Note: Some pipelines will require a reference sequence:
If you have the Manager role on a project, you can add a reference file to the project. Reference files added to a project can be shared with all other project members.
If you do not have the Manager role on a project, or you would just like to upload a reference file for this pipeline, you can add a new reference file to the analysis by clicking on the “ Upload New” button. If you upload a reference to the analysis instead of the project, no other users will be able to see or use the file you’ve uploaded.
When you select a pipeline that doesn’t require a reference file (or you’ve already uploaded one), you will be presented with a list of the samples that you’ve selected for analysis in the pipeline:
Removing samples from a pipeline submission
You can remove a sample from a pipeline submission by clicking on the “Remove” button on the right-hand side of the sample:
Multiple filesets per sample
If a sample has been sequenced multiple times (a top-up run, for example), the pipeline submission page will display multiple filesets for that sample:
You may only select one fileset for submission to the pipeline for analysis. You can select the fileset that you want to submit to the pipeline for analysis using the radio buttons on the left-hand side of the sample fileset display:
Modifying pipeline parameters
IRIDA has pre-defined some default values for pipeline submissions, but you may change the default parameters for your analysis by clicking on the “Customize” button on the right-hand side of the parameters drop-down at the top of the pipeline submission page:
The dialog window that appears will allow you to change the parameters for the pipeline execution:
If you would like to reset a value that you modified back to the default value, click on the “Use Default” button on the right-hand side of the paramater:
Note: customized pipeline parameters are not validated, please ensure that the values you are entering are valid for the parameter that you are modifying.
When you’ve finished customizing the pipeline parameters, you can click on the “Use these Parameters” button to return to the pipeline submission page.
Saving pipeline parameters
You may save the parameters that you modified by clicking on the “Save these parameters for later?” checkbox at the bottom of the parameters dialog:
You should provide a name for the pipeline parameters that reflects the modifications that you’ve made to the parameters. When you’ve entered the name for the parameter set, you can click on the “Use these Parameters” button at the bottom of the parameter modification dialog to return to the pipeline submission page.
Using pre-saved pipeline parameters
Parameter sets that have been saved can be used on subsequent executions of the pipeline by using the parameters drop-down, just below the pipeline name:
Running the pipeline
Your pipeline submission will be prepared and launched, and you will see a confirmation dialog:
You can monitor pipeline progress by clicking on the “Let’s see how this pipeline is doing” button, or you can empty your cart and start again by clicking on the “Let the pipeline run!” button.
Monitoring pipeline progress
The analysis overview list will show the current progress of all running and completed pipelines that you’ve submitted for execution:
The “State” column will change over time if you leave your browser window open so you can monitor the progress of your pipeline executions.
Viewing pipeline results
You can view or download the outputs of a pipeline execution once a pipeline has completed:
You can either click on the icon to download all of the outputs produced by the pipeline execution, or you can click on the name of the analysis to view more detailed outputs and, if available, view a preview of the outputs:
The analysis details page shows you more detailed information about your pipeline submission, including the names of the files that were produced by the analysis (on the left-hand side of the page), a preview of the outputs (if available), and some tabs to view more details about how the pipeline was submitted:
To download output files, you can use the “Output Files” section from this page. To download an individual file, click on the file name. To download all the outputs produced by the pipeline, you can click on the “Download Files” button.
Previewing analysis output files
All analysis pipelines produce analysis output files. You can preview these output files under the Preview tab on the Analysis Details page:
For each analysis output file, you will see a panel and in each panel you will see:
- a panel heading with the Galaxy tool name (e.g. “RefSeq Masher Matches”) and version (e.g. “(0.1.1)”), internal IRIDA output name (e.g. “refseq-masher-matches”) and output file name (e.g. “refseq-masher-matches.tsv”)
- a file download link
- a preview of the file contents displayed as plain text or in a table
Previewing tabular analysis output
Some of the output files will be rendered in a table:
When you scroll to the bottom row in the table, more lines will be fetched as they are needed. You can also resize the table by clicking and dragging the “resize icon” in the corner of the panel:
Default plain text preview of analysis output
Analysis output files with file extensions like
.fasta can be previewed in the browser as plain text:
If an analysis output file is small enough like this log file, it will be loaded in its entirety:
If an analysis output file is fairly large like this FASTA file, it will be loaded in chunks as needed for viewing:
Viewing the sequencing data submitted for analysis
To view the data that was submitted to the pipeline for analysis, start from the analysis details page and click on the “Input Files” tab:
The pipeline input files page shows all of the files that were submitted for analysis in the pipeline submission:
You can view more details about each of the files that were submitted in the pipeline submission by clicking on the names of the files. This provides you a quick way to view the file details, including quality control measurements provided by FastQC.
Viewing pipeline parameters used at runtime
To view the parameters that were used when submitting the pipeline, start from the analysis details page and click on the “Provenance” tab:
The provenance page shows a list of the files that were generated as outputs by the pipeline execution:
You can click on each of the file names to expand the complete listing of how the file was generated behind the scenes:
You can further inspect the parameters of each of the tools that were used to generate the analysis by clicking on one of the steps:
Edit analysis name
If you are the user who submitted the pipeline, you are able to change the submission name from the results page. Click the pencil icon next to the analysis name, enter the new analysis name, and click update.
Sharing pipeline results
Pipeline results can be shared with projects so that other users on a shared project can view results of the analysis.
Sharing when launching a pipeline
When launching a pipeline you can select which projects the results should be shared with by selecting them in the
Share Results section of the pipeline setup.
Note that for a project to be displayed in this list it must have samples being used in the pipeline.
Once the pipeline has been submitted the submission will show up in the analysis listing for the selected projects. From this page users on the project will be able to monitor the pipeline’s progress and view results once they are complete. For more information on viewing analysis results shared with a project, see the Managing Projects page.
Share results after pipeline has been launched
Pipeline results can also be shared anytime after a pipeline has been launched. To view or modify which projects an analysis has been shared with, click the
Share Results tab on the pipeline results page.
From this page you will be shown all projects that the pipeline result has been shared with (indicated by a check mark) along with additional projects available for sharing results. To share or unshare the results with a project, check or uncheck the box next to the project name.
Note that for a project to be displayed in this list it must contain samples which were used in the analysis.
Saving pipeline results to a sample
Some pipelines allow you to save data back to the samples in which the pipeline was ran. The type of data which will be saved back to the samples will differ between pipelines. For example an assembly and annotation pipeline will save the assembly to the list of files on a sample, whereas a typing pipeline such as SISTR may save a serotype to the metadata of the sample.
To save analysis results back to a sample, on submission of a pipeline please select the
Save Results to Samples checkbox on the pipeline setup page.
Results can also be saved after a pipeline has completed. To save analysis results after the pipeline has finished, click the
Share Results tab on the pipeline results page. If you are able to save the analysis results back to the sample, a
Save Results section will be displayed. Click the button to save the results to the related samples.
Downloading single sample analysis output files in batch
You can download all of your single sample analysis output files in batch by clicking Analyses>Output Files in the top navigation bar:
You will see a table of your single sample analysis output files:
You can apply filters to any columns by clicking the column menu icon, for example, applying a filter to the
Filtering for files with
contigs in the filename:
You will then see a filter icon displayed beside the column name
You can select the files you wish to download using the checkboxes or by holding the
Shift keys as you click on the rows for the files you wish to download.
Or you can click the checkbox in the leftmost column header to select all files displayed:
Click Download to download. Depending on the number of files you have selected, preparation of the download may take a moment.
Single files will be downloaded as is, while multiple files will downloaded bundled together in ZIP file.
Errors when executing pipelines
You may experience an analysis pipeline execution failure caused by an error in one or more tools within a pipeline.
For example, you may encounter an error when running the “Assembly and Annotation” pipeline:
If you click on the icon, you can preview the Galaxy job error information:
If you follow the link to the analysis page, you can view all the job error information for the pipeline in error:
This information may be helpful for troubleshooting and communicating what went wrong in a particular analysis pipeline.