Uploading with the Uploader Tool

This is a quick tutorial on how to upload data to IRIDA using the IRIDA MiSeq Uploader Tool. You should use the IRIDA MiSeq uploader tool if you’re uploading an entire MiSeq run to IRIDA. Usually you only want to use the uploader tool if you’re a sequencing facility doing sequencing on behalf of a client.

This tutorial assumes that your client has already created the project where they want their data to reside in IRIDA. Clients must tell you what the numeric identifier for their project is so that you can use that information when setting up the run in the Illumina Experiment Manager.

Downloading and Installing the Uploader

The Illumina MiSeq platform has a Windows 7 PC installed inside the sequencer. A Windows installer for the Illumina MiSeq uploader tool can be downloaded at: https://irida.corefacility.ca/downloads/tools/IRIDA_Uploader_latest.exe

You can safely accept all default options once you launch the installer.

What does the installer install?

For complete transparency, the installer will install several components required by the uploader:

Launching the Uploader

The installer will create a Start menu entry on your system:

Start menu entry

You can launch the uploader by clicking on the entry in the Start menu.

Configuring the Uploader

Creating a client in IRIDA

Before you can prepare the uploader to connect, you must create a client ID and secret in IRIDA.

Start by clicking on the “Admin” menu (in the top, right-hand corner of the screen) and selecting “Clients”:

Administrator clients menu.

The clients list shows all clients that are currently allowed to access the IRIDA REST API.

You can add a new client by clicking on the “Add Client” button:

Add client button.

When you add a client, you’ll be required to provide the following information:

  1. A unique client ID,
  2. How long a token should be valid for once issued,
  3. The type of OAuth2 flow that a client should use,
  4. Whether to allow refresh tokens.
  5. The scopes that the client is allowed to use (should the client be allowed to read, write, or both with the REST API).

Client details.

Important: When you’re creating a client entry for your uploader, you must select the password grant type, and you must select both the read and write scopes.

Configuring the Uploader Settings

Once you’ve created the client ID and secret in the IRIDA web interface, you need to enter that information into the settings dialog of the uploader. Open the settings dialog by clicking on the Options menu and selecting Settings:

Settings menu

The default settings for the uploader will not work with your IRIDA install:

Default settings dialog

On successful connection, your settings dialog should have green checkmarks on each setting:

Successful settings dialog

Modifying the Sample Sheet

You must enter the numeric project ID number into the sample sheet for every sample that’s going to be uploaded using the uploader tool. You can enter this number in the Illumina Experiment Manager tool in the Sample Project column:

Illumina Experiment Manager

You may also alter this value after the sequencer has completed the run using a spreadsheet tool like Microsoft Excel. Find the file named SampleSheet.csv in the directory containing the results of your experiment and open the file with Microsoft Excel. Enter the numeric project ID in the Sample_Project column for each sample in the list. Make sure that you save the file as a CSV file.

The uploader tool will create samples in the project(s) using the Sample ID field as the sample name. If a sample in the project already has a name in the Sample ID column (as might be the case with a top-up run), then the uploader will simply upload the new data to the existing sample.

Uploading Data

When you’ve finished configuring the uploader and modifying the sample sheet, you can proceed with upload.

Launch the uploader and choose the directory that contains your experiment results by clicking on the “Choose directory” button:

Choose directory

Use the dialog that appears to select the directory containing one or more sequencing run data directories, then click OK. You will see a success message indicating that the upload is ready to proceed if you’ve selected a directory that contains a valid SampleSheet.csv file:

A valid directory is selected

When you’re ready to proceed with uploading, click on the “Upload” button at the bottom of the window. The upload will begin, and you will see the progress of individual files and the overall run indicated at the bottom of the window, along with an estimated time remaining before the upload is complete:

Upload proceeding

Depending on the size of your experiment and your connection speed to your instance of IRIDA, upload can take several minutes to hours of time.