IRIDA Workflow Description

This file describes the IRIDA Workflow Description format. This is an XML format that is used to link up a Galaxy Workflow and the corresponding Galaxy Tools into IRIDA and provide IRIDA with the necessary information on the input datasets for Galaxy, parameters, and output files to save.

Workflow Description XML Element Details

<iridaWorkflow>

The outer-most element tag. All other tags are contained within an <iridaWorkflow>.

Attributes

None.

Example

<iridaWorkflow>
...
</iridaWorkflow>

<id>

An ID for the workflow which is used to uniquely identify this exact workflow. This should be a UUID. A quick way to generate a random (version 4) UUID is with the uuid command, as in uuid -v 4.

Attributes

None.

Example

<id>79872edb-4f3b-4a51-aff7-33ddbc05ec5e</id>

<name>

The name of the workflow.

Attibutes

None.

Example

<name>MyPipeline</name>

<version>

A version number for the workflow. This should be incremented on every modification to the workflow.

Attributes

None.

Example

<version>1.0</version>

<analysisType>

The particular type or class of analysis this workflow belongs to. This must be one of the types defined in the AnalysisType enum in IRIDA. Currently, the type can be either assembly-annotation or phylogenomics. Please see the AnalysisType JavaDoc for more details.

Attributes

None.

Example

<analysisType>phylogenomics</analysisType>

<inputs>

A description of the different types of inputs to this workflow. This must contain at least one of <sequenceReadsPaired> or <sequenceReadsSingle> as a sub-element.

Attributes

None.

Example

<inputs>
	<sequenceReadsPaired>sequence_reads_paired</sequenceReadsPaired>
</inputs>

<parameters>

This defines the set of parameters for a workflow as well as how to map these parameters to Galaxy tool names and versions.

Attributes

None.

Example

<parameters>
    <parameter name="myparameter" defaultValue="HKY85">
        <toolParameter toolId="irida.corefacility.ca/galaxy-shed/repos/irida/phyml/phyml1/3.1"
            parameterName="datatype_condition.model" />
    </parameter>
</parameters>

<outputs>

Used to define a list of output files from the Galaxy workflow which will be saved back into IRIDA.

Attributes

None.

Example

<outputs>
    <output name="tree" fileName="phylogeneticTree.tre" />
</outputs>

<toolRepositories>

Defines a list of the repositories storing the dependency Galaxy tools for this workflow. This is used to keep track of the particular versions of dependencies and can also be used for integration testing with Galaxy.

Attributes

None.

Example

<toolRepositories>
    <repository>
        <name>spades</name>
        <owner>lionelguy</owner>
        <url>https://toolshed.g2.bx.psu.edu/</url>
        <revision>21734680d921</revision>
    </repository>
</toolRepositories>

<inputs> Elements

<sequenceReadsPaired>

An optional element tag contained in the <inputs> tag set. This defines the name, if any, of a list of paired-end files used as input to the Galaxy workflow.

Attributes

None.

Example

If a Galaxy workflow contains an Input dataset collection of type list:paired then the name of the dataset collection, sequence_reads_paired, should correspond to the name in this element. Please see the image below.

Galaxy Workflow Input

galaxy-paired-input

Workflow Description File

<sequenceReadsPaired>sequence_reads_paired</sequenceReadsPaired>

<sequenceReadsSingle>

An optional element tag contained in the <inputs> tag set. This defines the name, if any, of a list of single-end files used as input to the Galaxy workflow.

Attributes

None.

Example

If a Galaxy workflow contains an Input dataset collection of type list then the name of the dataset collection, sequence_reads_single, should correspond to the name in this element. Please see the image below.

Galaxy Workflow Input

galaxy-single-input

Workflow Description File

<sequenceReadsSingle>sequence_reads_single</sequenceReadsSingle>

<reference>

An optional element tag contained in the <inputs> tag set. This defines the name, if any, of the reference input for the Galaxy workflow.

Attributes

None.

Example

If a Galaxy workflow contains an Input dataset for a reference genome, then the name of the dataset, reference, should correspond to the name in this element. Please see the image below.

Galaxy Workflow Input

galaxy-reference-input

Workflow Description File

<reference>reference</reference>

<requiresSingleSample>

An optional element tag contained in the <inputs> tag set. This defines whether or not this workflow only operates on a single sample true or can handle multiple samples false. That is to say, if this workflow will upload only a single sample to Galaxy to execute the workflow, such as with the assembly and annotation workflow, then this should be set to true. Otherwise this should be set to false. The default is false.

Attributes

None.

Example

<requiresSingleSample>false</requiresSingleSample>

<parameters> Elements

<parameter>

Contained in the <parameters> element tag. This defines a single parameter for a workflow. This must contain at least one <toolParameter> element which defines the specific Galaxy tool and parameter to override. The defaultValue should also correspond to one of the acceptible Galaxy parameter values.

Attributes

attribute type details required example
name string The name of the parameter. This will be used in the IRIDA database and configuration files to refer to this parameter. yes name="myparameter"
defaultValue string The default value of the parameter. yes defaultValue="1"

Example

To override the model parameter defined in the Galaxy version of PhyML in http://irida.corefacility.ca/gitlab/analysis-pipelines/snvphyl-galaxy/blob/v0.1/tools/phyml/phyml.xml, the following entry can be used. The defaultValue="HKY85" must correspond to the value defined in the Galaxy PhyML Tool (see phyml.xml#L38).

<parameter name="myparameter" defaultValue="HKY85">
    <toolParameter toolId="irida.corefacility.ca/galaxy-shed/repos/irida/phyml/phyml1/3.1"
        parameterName="datatype_condition.model" />
</parameter>

<toolParameter>

Contained in the <parameter> element tag. This defines a parameter in a Galaxy tool to map to from the parent <parameter> definition. The toolId and parameterName must correspond to the information defined in the Galaxy workflow and Galaxy tool XML configuration files.

Attributes

attribute type details required example
toolId string The id of the tool in the Galaxy workflow of the parameter to override. yes toolId="my_galaxy_tool"
parameterName string The name of the parameter in the Galaxy tool to override. yes parameterName="parameter.section.name"

Example

For the tool PhyML in the SNVPhyl Galaxy Workflow, the tool_id is given below:

tool_id (see snvphyl-workflow.ga#L506)

"tool_id": "irida.corefacility.ca/galaxy-shed/repos/irida/phyml/phyml1/3.1"

This corresponds to the toolId attribute to use in the <toolParameters> element (tool_id="irida.corefacility.ca/galaxy-shed/repos/irida/phyml/phyml1/3.1").

For the model parameter name, the corresponding line in the SNVPhyl Galaxy workflow is given below:

model (see snvphyl-workflow.ga#L507)

"tool_state": "...\\\"model\\\": \\\"HKY85\\\"...

However, this parameter is stored as a sub-element of other parameters. To get the full parameter name the PhyML Galaxy Tool XML file must be referenced:

parameterName (see phyml.xml#L6)

<command interpreter="bash">./phyml.sh ... -m $datatype_condition.model ...
...
</command>

Since the string datatype_condition.model is used to refer to the model parameter, this should be used as the full parameterName attribute.

Putting both these together into the IRIDA Workflow Description <toolParameter> element gives:

<toolParameter toolId="irida.corefacility.ca/galaxy-shed/repos/irida/phyml/phyml1/3.1"
	parameterName="datatype_condition.model" />

<outputs> Elements

<output>

Contained in the <outputs> element tag. Defines a particular output file from a Galaxy workflow. In order to save this output file in IRIDA, the name of the dataset file in Galaxy must be defined in the fileName attribute of the <output> element. This can be accomplished by using the Rename Dataset action in a Galaxy workflow to give an output dataset file an explicit name.

Attributes

attribute type details required example
name string A label for the output file name. This is used internally to map to a particular output file in the IRIDA database. yes name="my-output-1"
fileName string The name of an output file from the Galaxy workflow. This is used to find the particular output file when transferring results back to IRIDA. yes fileName="output-file.txt"

Example

If the Rename Dataset action is set on a tool to give an output file the name phylogeneticTree.tre

galaxy-workflow-action-output

then the corresponding dataset will appear with this name in the Galaxy History.

galaxy-workflow-dataset-output

This name must then correspond to the fileName in the workflow description file.

Workflow Description File

<output name="tree" fileName="phylogeneticTree.tre" />

<toolRepositories> Elements

<repository>

Contained within a <toolRepositories> element tag. Defines a particular repository storing a dependency for the workflow. This must correspond to the information within some Galaxy ToolShed containing this repository. For example, for SPAdes https://toolshed.g2.bx.psu.edu/view/lionelguy/spades/21734680d921.

Attributes

None.

Example

For the version of SPAdes stored within the main Galaxy ToolShed, https://toolshed.g2.bx.psu.edu/view/lionelguy/spades/21734680d921, the following information should be defined.

<repository>
    <name>spades</name>
    <owner>lionelguy</owner>
    <url>https://toolshed.g2.bx.psu.edu/</url>
    <revision>21734680d921</revision>
</repository>

<name>

Contained in the <repository> element tag. Defines the name of the tool repository.

Attributes

None.

Example

For SPAdes, https://toolshed.g2.bx.psu.edu/view/lionelguy/spades/21734680d921, the name would be spades.

<name>spades</name>

<owner>

Contained in the <repository> element tag. Defines the owner of the tool repository.

Attributes

None.

Example

For SPAdes, https://toolshed.g2.bx.psu.edu/view/lionelguy/spades/21734680d921, the owner would be lionelguy.

<owner>lionelguy</owner>

<url>

Contained in the <repository> element tag. Defines the url of the tool repository.

Attributes

None.

Example

For SPAdes on the main Galaxy ToolShed, https://toolshed.g2.bx.psu.edu/view/lionelguy/spades/21734680d921, the url would be https://toolshed.g2.bx.psu.edu/.

<url>https://toolshed.g2.bx.psu.edu/</url>

<revision>

Contained in the <repository> element tag. Defines the revision number of tool.

Attributes

None.

Example

For the version of SPAdes on the main Galaxy ToolShed, https://toolshed.g2.bx.psu.edu/view/lionelguy/spades/21734680d921, the revision would be 21734680d921.

<revision>21734680d921</revision>

Workflow Description Example

An example workflow description XML file is given below.

<?xml version="1.0" encoding="UTF-8"?>

<iridaWorkflow>
        <id>79872edb-4f3b-4a51-aff7-33ddbc05ec5e</id>
        <name>MyPipeline</name>
        <version>1.0</version>
        <analysisType>phylogenomics</analysisType>
        <inputs>
                <sequenceReadsPaired>sequence_reads_paired</sequenceReadsPaired>
                <reference>reference</reference>
                <requiresSingleSample>false</requiresSingleSample>
        </inputs>
        <parameters>
            <parameter name="myparameter" defaultValue="HKY85">
                <toolParameter toolId="irida.corefacility.ca/galaxy-shed/repos/irida/phyml/phyml1/3.1"
                    parameterName="datatype_condition.model" />
            </parameter>
        </parameters>
        <outputs>
                <output name="tree" fileName="phylogeneticTree.tre" />
        </outputs>
        <toolRepositories>
            <repository>
                <name>spades</name>
                <owner>lionelguy</owner>
                <url>https://toolshed.g2.bx.psu.edu/</url>
                <revision>21734680d921</revision>
            </repository>
            <repository>
                <name>phyml</name>
                <owner>irida</owner>
                <url>https://irida.corefacility.ca/galaxy-shed</url>
                <revision>b5867c5c7674</revision>
            </repository>
        </toolRepositories>
</iridaWorkflow>